Hepatic Fibrosis/Liver Fibrosis

Hepatic fibrosis refers to liver injury, characterized due to excessive production and deposition of extracellular matrix (ECM) molecules. The advanced stage characterized by widespread fibrous scarring called cirrhosis. Experimental animal models of hepatic fibrosis provide significant tools to understand the cellular and molecular mediators of fibrosis in a various manner during both progression and recovery. Several approaches to induction of fibrosis have been developed. Among these, CCl4 and TAA intoxication in rats and mice are the most widely used method for induction of hepatic fibrosis. CCl4 and TAA models best characterized with respect to histological, biochemical, cell, and molecular changes associated with the development of fibrosis.

Invitek offers several hepatic fibrosis models in rats and mice for screening, testing, and evaluation of new drugs and formulations.

Hepatic Fibrosis Model-Induced By CCl4 In Rats and Mice

Hepatic fibrosis induced by Intraperitoneal (i.p.) injection of CCl4 in rats and mice. Following acclimation, animals assigned to different groups according to their body weight and injected CCl4 intraperitoneally (i.p.) mixed with Olive oil/corn oil/mineral oil twice or thrice weekly for 4-12 weeks. Test articles dosed via either of the routes of the administration [PO, IP, and SC] for 4-16 weeks.

Hepatic Fibrosis Model-Induced By TAA In Rats and Mice

Thioacetamide (TAA) is another well-established model of experimental liver fibrosis in rodents. Hepatic fibrosis induced by intraperitoneal (i.p.) injection of TAA or orally through drinking water in rats or mice. TAA-reversal serves as a good model to test potential antifibrotics.

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Non-Alcoholic Fatty Liver Disease (NAFLD)

Non-Alcoholic fatty liver disease (NAFLD) represents a spectrum of liver disease associated with fibrosis, cirrhosis, steatohepatitis and hepatocellular carcinoma. NAFLD also associated with obesity, diabetes, and insulin resistance. Genetically and dietary manipulated animal models best-characterized the models of NAFLD.

Invitek offers both genetic and dietary manipulated mouse and rat models of NAFLD

Genetic Models

  • Leptin-deficient (ob/ob) mouse model
  • Leptin-resistant (db/db) mouse model

Dietary Models

  • Methionine and choline-deficient model,
  • High-fat diets (HFD) model